General inhibitors and inhibition mechanism

General inhibitors and inhibition mechanism

General inhibitors and inhibition mechanisms can cause enzyme activity to decrease, but do not degrade the enzyme, can be called enzyme inhibitors. There are many types of inhibitors, inorganic and organic, low molecular and high molecular, specific and non-specific. The effect caused by inhibitors is called inhibition, or inhibition for short. After the inhibitor is combined with the enzyme, you can add other substances and use other methods, such as dialysis to remove the inhibitor to inhibit the enzyme activity recovery, called reversible inhibition. The inhibitor is combined with the enzyme by a relatively strong covalent bond to inactivate the enzyme. The inhibitor cannot be removed by dialysis and other methods, so that the inhibition of the enzyme's recovery activity is called irreversible inhibition. In immunoenzyme technology, it is necessary to fully inhibit the relevant endogenous enzymes in the tissue, but also to preserve the relevant antigen activity. There are various inhibition mechanisms, for example, it can form a stable complex with the metal ion in the enzyme to inactivate the enzyme, or combine with some free vegetable groups of the enzyme to change the enzyme. Substances that have inhibitory effects on iron-containing porphyrinases, in addition to azide compounds, also include cyanide, hydrogen sulfide, etc. The inhibitors that are studied more in enzyme chemistry are heavy metal ions, fluorides, and organic positive ions (such as Alkaloids and dyes, etc.), iodoacetic acid and ethylenediaminetetraacetic acid. These counteractants respectively inhibit different enzymes. In immunoenzyme technology, it is necessary to fully inhibit the relevant endogenous enzymes in the tissue, but also to preserve the relevant antigen activity. Research on the inhibition of endogenous enzymes in tissues. It is helpful for the correct positioning of antigens in tissues by immunoenzyme technology.

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