1. Experimental conditions Cometro (US Connaught) high performance liquid chromatography system; Cometro (US Connaught) 6000 LDI Pump; Softa (Sofoda USA) ELSD (evaporative light scattering detector), DT-70 ℃, SC-30 ℃; Scienhome (Tianjin Chenhang) Kromasil NH2 column; Mobile phase: acetonitrile-water = 70-30; Flow rate: 1.0ml / min; Lactose standard concentration is 0.5mg / mL, 1mg / mL, 1.5mg / mL; Sample configuration of whey powder: Take 0.1g of powder and put it in a 100mL volumetric flask, and bring the volume to the mark with water. The injection volume is 20ul. Second, the experimental results Sample one: Detector 1 Results Retention Time Area Theoretical plates (USP) Resolution (USP) 2.698 104649 1083 0.00000 16.797 3671699 5914 23.46733 Sample two: Detector 1 Results Retention Time Area Theoretical plates (USP) Resolution (USP) 2.695 149923 792 0.00000 16.871 3700293 5739 22.25627 Sample three: Detector 1 Results Retention Time Area Theoretical plates (USP) Resolution (USP) 2.688 133156 845 0.00000 16.821 3733804 5535 22.18048 Lactose standard (inject three times at different concentrations, and reinject 2 times at a concentration of 1 mg / ml for reproducibility) Relationship between concentration and peak area: concentration Peak area Unknown (Sample 1) 3671699 Unknown (Sample 2) 3700293 Unknown (sample three) 3733804 Standard product 0.5mg / mL 2288817 Standard product 1.0mg / mL 4865288/4844636/4797327 RSD = 0.72% Standard product 1.5mg / mL 7750119 Take lg concentration as abscissa and lg peak area as ordinate Bring the concentration of the sample into the three sample concentrations that can be calculated by the equation and the mass percentage relative to the powder: Concentration mg / mL Mass percentage Sample one 0.769619 76.96% Sample two 0.775031 77.50% Sample three 0.782605 78.26% Three, discussion of results It can be seen from the figure that the chromatographic column has a good peak shape for the sample, high column efficiency, and good reproducibility of the Softa ELSD (evaporative light scattering detector) (RSD = 0.72%) with a good linear relationship R2 = 0.999). Coat Hooks,Wall Hooks,Hanging Hooks,Pintle Hitch Fungho Industries (Group) Co., Limited , https://www.funghogroup.com
ELSD (evaporative light scattering detector) analysis report of lactose
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