Common microorganisms in water and wastewater and sewage treatment are: Bacteria, Fungi, Algae, Protozoa, Rotifers, Crustaceans, Worms (Nematodes and Flatworms). The main testing items are the total number of bacteria, the total number of yeasts, the total number of molds, and the total number of E. coli. 1. Determination of the total number of bacteria 1.1 After diluting 103 times, 104 times, 105 times, 106 times, and 107 times of the water samples in the biochemical pool, take 1 mL of each dilution in a Petri dish, pour the plates, and incubate in a biochemical incubator at 37 ° C for 24 hours, then select colonies Count the cultures between 30 and 300, and make 5 parallel samples for each dilution factor. 1.2 Take a 100mL water sample bath until a small amount of water remains, bake in a 105 ° C oven to constant weight, weigh the dry weight of the activated sludge in the water sample, and calculate the total number of bacteria per gram of dry sludge in the biochemical pool water sample. 2. Determination of the total number of yeasts 2.1 After the water samples in the biochemical pool were diluted 102-fold, 103-fold, 104-fold and 105-fold, 1mL of each dilution was inoculated into a Petri dish, poured into plates, and cultured in a biochemical incubator at 30 ° C for 48h, and the number of colonies was selected at 30 Count between ~ 300 cultures and make 5 replicates for each dilution factor. 2.2 Take a 100mL water sample in a water bath until a small amount of water remains, and bake it to a constant weight in an oven at 105 ° C. Weigh the dry weight of the activated sludge in the water sample and calculate the total number of yeasts per gram of dry sludge in the biochemical pool water sample. 3. Determination of the total number of molds 3.1 After diluting the water samples in the biochemical pool by 102 times, 103 times, 104 times and 105 times, inoculate 1 mL of each dilution in a petri dish, pour the plates, and incubate in a biochemical incubator at 30 ℃ for 72 hours, then select the number of colonies at 30 Count between ~ 300 cultures and make 5 replicates for each dilution factor. 3.2 Take a 100mL water sample in a water bath until a small amount of water remains, and bake in a 105 ° C oven to constant weight, weigh the dry weight of the activated sludge in the water sample, and calculate the total number of molds per gram of dry sludge in the biochemical pool water sample. 4. Determination of the total number of coliform bacteria 4.1 Initial fermentation test: Aseptically operate 5 fermentation tubes each containing 5 ml of 3 times concentrated lactose peptone culture solution, inoculate 10 ml of sewage samples each, and each contain 5 tubes of single lactose peptone culture solution Inoculate 1 ml of sewage sample in each fermentation tube, and then inoculate 1 ml of 1:10 diluted sewage sample (equivalent to 0.1 Ml). Place the 15 inoculated fermentation tubes in a 37 ° C incubator and incubate for 24 hours. 4.2 Plate separation: After culturing for 24 hours, inoculate the fermentation tubes that produce acid and gas and only produce acid and no gas, and inoculate them on echinacea culture medium or magenta sodium sulfite culture medium respectively. ~ 24 hours, select colonies that meet the following characteristics, take a small part of the colonies, smear, Gram stain, and microscopic examination. In the colony total inspection work, you will encounter the colony counting work of various experimental plates, such as the pouring method plate, the spiral inoculation plate, the 3M colony total test paper, and the filter method plate. Moreover, manual counting consumes both time and manpower. The statistics increase with people's fatigue, and some workers even have a hurry to reduce the workload. Xunshuo automatic colony counter has the accurate statistical function of different types of plates, and can save plate images and databases for viewing and verification. Xunshuo colony analysis instrument adopts colony enlarged image shooting, high-resolution 10 million pixel CCD digital Imaging technology and Colonfast colony intelligent identification technology. 1. Determination of the total number of bacteria In terms of automatic colony counting, the instrument can quickly complete all kinds of pouring method plates, coating method plates, filter method plates, air sedimentation method plates, spiral inoculation plates, and 3M paper colonies containing samples of bacteria, mold, yeast, etc. Automatic counting and concentration calculation. The speed of automatic counting colonies is higher than 500 colonies / second, the smallest resolvable colony diameter can reach 0.01mm; a special counting template can be constructed to adapt to the complex laboratory environment, and the counting error is controlled within 10%. 2. Against yeasts and molds The Xunshu colony analysis instrument has a built-in special algorithm, which supports the automatic counting and concentration calculation of colonies of various pouring method plates, coating method plates, filter method plates, and spiral inoculation plates containing samples of mold and yeast. 3. Against E. coli The automatic colony counting ability of Xunshuo instrument can help experimenters to quickly and accurately select "VRBA tablet with 30 to 150 colonies"; using color high-pixel CCD imaging and Colonfast image intelligent recognition software, Xunshuo colony instrument can quickly identify "purple" The colonies of the coliform bacteria in red, with a precipitation ring, and a diameter of not less than 0.5 mm "can be counted in only 1 second. 4. Some special sewage also contains highly pathogenic pathogenic microorganisms, such as Bacillus subtilis var. Spores. Detection method: 1) Take an appropriate amount of spore suspension of Bacillus subtilis black variant and mix it into the collection tank to make the final concentration of bacterial solution in the sewage reach (2 ～ 8) × 104 / mL 2) Perform chemical and physical disinfection in sequence in accordance with the sewage treatment procedures. 3) The filter membrane is boiled and sterilized three times in distilled water for 15 min each time; the filter is sterilized with pressure steam (121 ° C., 20 min). 4) Detection and sampling: After the disinfection process is completed, use a graduated pipette to draw 100mL of sewage to be discharged from the sampling port of the sewage collection tank, and filter with a microporous filter membrane under sterile conditions. 5) The filter membrane is washed with 50 mL of PB buffer solution to remove residual disinfectant. 6) Collect the washing solution and filter it with a microporous membrane under sterile conditions. 7) Put the microporous membrane under sterile conditions on a pancreatic soybean agar plate for bacterial culture. The filter membrane should be completely in close contact with the culture medium, and no air bubbles should be left in it. 8) Invert the sampling plate in the incubator at 35 ± 0.5 ℃ for 24 ± 2 hours, and count the Bacillus subtilis colony. For colony counting of filter membranes, 3M paper sheets, etc., Xunshuo's Colonfast colony intelligent identification technology can accurately identify colony characteristics and achieve rapid automatic counting. Related Links: Hangzhou Xunshu Technology Co., Ltd. is a high-tech company specialized in microbial analysis and testing technology research and equipment production. At present, it mainly produces automatic colony counters, spiral inoculation microbial colony analyzers, bacteriostatic circle measuring instruments, β-lactam analyzers, antibiotic potency analyzers, algae counters, algae auxiliary identification systems, zooplankton counters, biological Micro analysis system and other products. Products are widely used in scientific research, inspection and quarantine, quality supervision, environmental monitoring, disease control center, drug and biological product verification, food and pharmaceutical daily chemical production, and iron and petrochemical chemical power. At present, the company's products have become the Chinese Academy of Sciences, the Chinese Academy of Agricultural Sciences, the Chinese Academy of Inspection and Quarantine, the Chinese Institute of Pharmaceutical and Biological Products, the China Animal Health and Epidemiology Center, the National Aquatic Products Quality Supervision and Inspection Center, the State Oceanic Administration, as well as Shanghai Baosteel and Lanzhou The first choice for thousands of domestic enterprises and institutions such as Petrochemical. And successfully served the drug resistance monitoring of the Australian AMS laboratory, the water quality test of the Veolia Water Group in France, the bacterial resistance analysis of the Royal DSM Group in the Netherlands, the toxicology center test of the WuXi PharmaTech, and the Pfizer Pharmaceuticals of the United States. The bacteriostatic circle (antibiotic potency) automatic detection and other occasions. The company's products and excellent after-sales service are widely praised and welcomed at home and abroad. PTFE Fabric Belt is made of PTFE Coated Fabrics. Jiangsu Youngsun New Materials Co.Ltd choose high strength glassfiber yarn and woven into fabrics ourselves. Then coated with special formula PTFE, which ensure the material to have super nonstick surface. What's more, it can resist high temperature up to 360 degrees. 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Application of Fully Automatic Colony Counter for Sewage Microbe Detection