Cas number 40567-80-4
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Molecular formula C12H18NNaO3S
Molecular weight 279.33
Alias ​​N-ethyl-N- (3-sulfopropyl) -3-methylaniline sodium salt (TOPS) N-Ethyl-N- (3-sulfopropyl) -3-methylaniline sodium s
White crystal powder.
Purity assay≥98% (T)
Moistrue ≤5%
Iron (Fe) <5PPM
Heavy Metals <5PPM
pH (5% in DI H2O) 6-8
Residue On Ignition ≤0.1%
Solubility 500mg / ml water molar absorption coefficient Molar Absorptivity 3.74 * 104 (oxidized product) at room temperature, protected from light
Product use Enzymatic spectrophotometric determination of water-soluble reagents for hydrogen peroxide.
The new Trinders reagent is a highly water-soluble aniline derivative, which is widely used in diagnostic tests and biochemical tests. It has several advantages over conventional color-generating reagents in the colorimetric determination of hydrogen peroxide activity. The new Trinders reagents are stable enough to be used in both solutions and test line inspection systems. In the presence of hydrogen peroxide and peroxidase, the new Trinders reagent during the oxidative coupling reaction with 4-aminoantipyrine (4-AA) or 3-methylbenzothiazolone hydrazone (MBTH) Forms a very stable purple or blue dye. The molar absorbance of the coupling dye with MBTH is 1.5-2 times higher than that with 4-AA; however, the 4-AA solution is more stable than the MBTH solution. The substrate is enzymatically oxidized by its oxidase to produce hydrogen peroxide. The concentration of hydrogen peroxide corresponds to the substrate concentration. Therefore, the amount of substrate can be determined by the color of the oxidative coupling reaction. Glucose, alcohol, acyl-CoA and cholesterol can be used to detect those substrates coupled with the new Trinders reagent and 4-AA. There are 10 new Trinders reagents. Among the new Trinders reagents, TOOS is the most commonly used. However, for specific substrates, testing different types of new Trinders reagents is necessary to develop the best detection system.
N-ethyl-N- (3-sulfopropyl) -3-methylaniline sodium salt