What should you pay attention to when storing and transporting biological reagents?

What should you pay attention to when storing and transporting biological reagents?

What should you pay attention to when storing and transporting biological reagents?

1. Dry ice is solid carbon dioxide, the temperature is below 78 ° C, wet ice is the temperature of ice, generally the temperature of the frozen condition, such as the temperature of ice frozen at minus 20 ° C is minus 20 ° C. For some protein products such as enzymes, dry ice transportation is required, and most other biochemical reagents can be wet ice;

2. When transporting normal temperature reagents, no ice is needed. For reagents that need to be refrigerated, when transporting with ice, there should be a separator between the reagent and the ice. It should not be directly in contact with the reagent package. For example, if a reagent is added to the outside of the reagent box, ice is added to the outside of the box. Basically, ice cubes are used. Ice formed directly from water, because the ice will form water after it melts, which easily contaminates the reagents. The amount of ice added is not strictly limited, and it is preferable to surround the reagent with ice cubes;

3, do not underestimate the amount of biological reagents, many antibody reagents, enzyme reagents are very expensive, can reach thousands of yuan / mg (that is, hundreds of thousands per kilogram, tens of thousands of yuan), its storage conditions are also very strict Otherwise it is easy to lose activity. Ice is recommended instead of crushed ice during transportation. The main reasons are: (1) the temperature of the crushed ice is high and the temperature of the ice is lower; (2) the ice can keep the temperature for a longer period of time; (3) Ice is easily converted into water.

Biological reagent access rules:

The solid powder reagent can be taken with a clean horn spoon. To take a certain amount of solids, place the solids on paper or on a watch glass and weigh on a scale. For accurate weighing, the weighing bottle is weighed on the balance. The liquid reagent is usually measured by a measuring cylinder. The volume of the measuring cylinder is 5 mL, 10 mL, 50 mL, 500 mL, etc., and the liquid to be measured is injected into the measuring cylinder during use, so that the line of sight and the lowest point of the liquid concave surface in the measuring cylinder are kept horizontal, and then read. The scale on the measuring cylinder is the volume of the liquid. If a small amount of liquid reagent is needed, it can be taken with a dropper. When taking it, care should be taken not to touch or insert the dropper onto or into the wall of the receiving container.

In order to achieve accurate experimental results, the following rules should be followed when taking reagents to ensure that the reagents are not contaminated and degraded:

(1) The reagent cannot be in contact with the hand.

(2) To use a clean medicine spoon, a measuring cylinder or a dropper to take reagents, it is absolutely not allowed to use the same tool at the same time and continuously use a variety of reagents. After taking one reagent, the tool should be washed (the spoon should be dried) before another reagent can be used.

(3) After the reagent is taken, be sure to close the stopper. Do not put the wrong bottle cap and the dropper. Do not allow the crown to be worn. After use, put the bottle back to the original position.

(4) The removed reagent can no longer be returned to the original reagent bottle.

In addition, the reagent should be used in a spirit-saving manner and used as little as possible, which is convenient for operation and careful observation of the phenomenon, and can obtain better experimental results.

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