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Human Epidermal Growth Factor (EGF) Enzyme Linked Immunoassay Kit Instructions for Use
Detection range: 96T 25pg/ml-480pg/ml Purpose of use: This kit is used to determine the epidermal growth factor (EGF) content of human serum, plasma and related liquid samples. Experimental principle This kit uses the double antibody sandwich method to determine the level of human epidermal growth factor (EGF) in the specimen. The microplate was coated with purified human epidermal growth factor (EGF) antibody to prepare a solid phase antibody, and epidermal growth factor (EGF) was sequentially added to the microcapsule of the coated monoclonal antibody, followed by HRP-labeled epidermal growth factor (EGF). The antibody binds to form an antibody-antigen-enzyme-labeled antibody complex, which is thoroughly washed and then added to the substrate TMB for color development. TMB is converted to blue under the catalysis of HRP enzyme and converted to the final yellow color by the action of an acid. The depth of the color is positively correlated with the epidermal growth factor (EGF) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human epidermal growth factor (EGF) in the sample was calculated from a standard curve. Kit composition 1 30 times concentrated washing solution 20ml × 1 bottle 7 Stop solution 6ml × 1 bottle 2 enzyme standard reagent 6ml × 1 bottle 8 standard product (960pg / ml) 0.5ml × 1 bottle 3 enzyme label coating plate 12 holes × 8 strips 9 standard dilution 1.5ml × 1 bottle 4 sample dilution 6ml × 1 bottle 10 instructions 1 part 5 color reagent A liquid 6ml × 1 bottle 11 sealing film 2 sheets 6 coloring agent B liquid 6ml × 1 Bottle 12 sealed bag 1