Abstract: Capsaicin ((trans) 8-methyl-N-vanillyl-6-nonenamide) is the active ingredient of pepper. It is irritating to mammals including humans and can produce a burning sensation in the mouth. Capsaicin and some related compounds are called capsaicin, and they are secondary metabolites produced by capsicum. 1 Scope: Method for determining capsaicinoids in chili peppers and chili products and expression of spiciness by high performance liquid chromatography. This standard applies to the determination of capsaicinoids in peppers and pepper products and the expression of spiciness. The detection limit of this method: capsaicin and dihydrocapsaicin are both 1mg / Kg, and the linear range is 1mg / L-150mg / L. 2. Sample extraction: Add 25ml of methanol-tetrahydrofuran mixed solvent to the sample, seal with plastic wrap, pierce a few small holes with a needle, and then extract under a 60 degree water bath condition using an ultrasonic shaker for 30min. Filter with filter paper, collect the filtrate, then add 25ml of methanol-tetrahydrofuran mixed solvent to the filter residue together with the filter paper, extract for 10min using an ultrasonic shaker, repeat twice, combine the filtrate collected by Xinchang Filter after three steps, and use Concentrate to 10ml-20ml at 70 ° C, then dilute to 50ml with a mixed solvent of methanol-tetrahydrofuran, filter through a 0.45µm filter membrane and perform chromatographic analysis. According to the total amount of capsaicinoids in the sample and the sensitivity of the detector, the dilution factor can be adjusted appropriately if necessary. 3. Reference conditions for chromatography: Lanbo column: KromasilC-18 (4.6mm * 250mm5um) Mobile phase: methanol: water = 65: 35 Injection volume: 10µl Flow rate: 1.0ml / min UV detection wavelength: 280nm Column oven temperature: 30 ℃ 4. Results: According to the chromatographic conditions, HPLC analysis was performed, and the retention time of the chromatographic peaks of the standard substances was used for qualitative analysis; according to the standard curve of capsaicin and dihydrocapsaicin and the peak areas in the samples were quantified. This rising Tv Lift is intended to keep your television out of site, either behind a console or bureau, or just in its lowered state. It can also be installed behind fireplace mantles, sideboards, credenzas, and nearly anywhere else. This sturdy TV lift can lift up to 39" from a fully lowered position (at 0.98"/second). All of this is controlled by a convenient wireless RF remote control. Motorized TV Lift Motorized Tv Lift,Desk Tv Lift,Motorized Lcd Tv Lift,Motorized Mobile Tv Lift Shaoxing contuo Transmission Technology Co.,Ltd , https://www.electricdesk.nl
Liquid Chromatographic Determination of Capsaicinoids in Pepper and Pepper Products