Various drawing software can be used to draw ELISA standard curve. The following uses "Curve Expert1.4" software as an example, the method of drawing ELISA standard curve is as follows;

1. Start "Curve Expert1.4" (Curve Expert1.4 free download.zip

2. Enter the OD value of the standard product on the X axis and the corresponding concentration value on the Y axis, as shown in the figure:

3. Click [Run]

Button, the following dialog box appears

4. Click the [ok] button, the following two dialog boxes appear, close the next dialog box

5. Some curve names appear in the upper right corner of the dialog box, click on the curve names in sequence starting from "1", and the corresponding fitted curve will appear in the lower right corner.

6. Double-click the curve with the best ELISA fit according to the fitted curve. Double-click and the following dialog box will appear:

Note: Select the curve equation with the best coefficient (ie "r" value) to perform the calculation. In the following dialog

There is an "r" value in the upper right corner

, The closer the "r" value to 1, the better the fit

7. Press the [Ctrl] key + [L] key, the following dialog box appears:

8. Enter the standard OD value, click [Calculate] and press to get the actual content of the protein to be tested (the specimen is diluted N times, the calculated value should be multiplied by N).

9. If you want to get the equation of the ELISA fitting curve, you can right-click in the blank space of the dialog box in step 6 and select "Information"

10. Get the following dialog box: Click "Copy"

Paste in the location you need to get the following data:

Rational Function: y = (a + bx) / (1 + cx + dx ^ 2)

Coefficient Data:

a = -21.531017

b = 212.05019

c = -0.24054479

d = 0.018060778

Tissue Roll,Roll Papers,Facial Tissue,Cottonelle Toilet Paper

ShanDong YongFang Sanitary Products Co.,Ltd , https://www.sdyongfang.com