The ELISA kit performs ELISA experiments strictly in accordance with the experimental steps. When the ELISA kit obtains the test data, it is necessary to process the calculated data, make a fitting curve, and calculate the concentration. You can use professional curve making software for analysis, such as curve expert 1.3, find the corresponding concentration from the standard curve according to the OD value of the sample, and then multiply it by the dilution factor; or use the standard concentration and OD value to calculate the regression equation of the standard curve , Substitute the OD value of the sample into the equation, calculate the sample concentration, and then multiply it by the dilution factor, which is the actual concentration of the sample. Here is a description of the calculation method using the ELISA kit EXCEL. You only need to double-click the chart and input it into the data of the chart to fit the curve. According to the curve equation, the concentration is calculated. method: 1. Fitting curve: Enter the first line: the concentration value, such as 0 10 50 100 400 Enter the second line: the adjusted od value at this concentration, such as 0 0.586 1.397 1.997 3.42 Select the input data, use the inserted chart button to enter the chart wizard, select "xy scatter chart" in "standard type"; select "polyline scatter chart" in "sub-chart type", and press "Next" "; Select" Series generation in line ", ELISA kit, press" Next "; data sign, you can fill in: such as data y-axis, OD value; data x-axis, concentration; press Next, click Finish. Available graphs. Click the curve, right-click, select "Add Trend Line", in the type, select the polynomial; in the options, select the display formula, choose to display the R squared value. Get the formula and R-squared value. You can also use the method described above: Double-click the chart and enter it into the chart data to fit the curve. 2. Calculate the concentration: The first experiment: The standard curve is: y = -4E-05x2 + 0.026x R2 = 0.9745 For example, the OD value is known, and the ELISA kit calculates the concentration. Since y = -4E-05x2 + 0.026x, you can get: 4E-05x2 -0.026x + y = 0 ax2 + bx + y = 0 Made of supreme quality EVA material, water resistant, moisture proofing and dust-proof. Lens Case,Eva Lens Case,Eva Contact Lens Case,Eva Hard Shell Lens Case Shenzhen Guohui Craft Products Co.,Ltd. , https://www.evacarrycase.com
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Calculation and processing method of experimental data of ELISA kit
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